Worksheet Chapter 13: Genetic Technology

1. Describe step-by-step how human insulin is produced commercially using recombinant DNA technology in host bacteria.

2. Explain how gel electrophoresis separates DNA fragments during DNA profiling.

3. True or False: Golden Rice is genetically engineered to express high levels of beta-carotene to combat Vitamin A deficiency.

4. Define 'Genetically Modified Organisms' (GMOs) and list two examples of GM crops.

5. State the functions of restriction enzymes and DNA ligase in recombinant DNA technology.

6. Which technology is used to restore oil-polluted marine ecosystems using oil-degrading bacteria?

7. Discuss two potential benefits and two bioethical concerns regarding the widespread adoption of Genetically Modified Foods (GMFs).

8. Fill in the blank: Enzymes that join foreign DNA fragments with plasmid DNA by forming phosphodiester bonds are called DNA ________.

9. True or False: Polymerase Chain Reaction (PCR) is used to separate DNA fragments according to their molecular size.

10. Outline three key steps involved in gene therapy for treating a genetic disorder.